Chat with tutor

Ask Questions, Get Answers

Questions  >>  CBSE XII  >>  Biology

Two persons filed a case against a lady claiming to be the father of her only daughter. How to identify the real biological father?

1 Answer

The real father can be identified by DNA finger printing technique.
* VNTR (variable number of tandem repeats) are satellite DNAs that show high degree of polymorphism.
* VNTRs are used as probes in DNA fingerprinting.
* First of all, DNA from an individual is isolated and cut with restriction endonucleases.
* Fragments are separated according to their size and molecular weight on gel electrophoresis.
* Fragments separated on electrophoresis gel are blotted (immobilised) on a synthetic membrane such as nylon or nitrocellulose.
* Immobilised fragments are hybridised with a VNTR probe.
* Hybridised DNA fragments can be detected by autoradiography.
* VNTRs vary in size from 0.1 to 20 kb.
* Hence, in the autoradiogram, band of different sizes will be obtained.
* These bands are characteristic for an individual. They are different in each individual, except identical twins.
Help Clay6 to be free
Clay6 needs your help to survive. We have roughly 7 lakh students visiting us monthly. We want to keep our services free and improve with prompt help and advanced solutions by adding more teachers and infrastructure.

A small donation from you will help us reach that goal faster. Talk to your parents, teachers and school and spread the word about clay6. You can pay online or send a cheque.

Thanks for your support.
Please choose your payment mode to continue
Home Ask Homework Questions
Your payment for is successful.
Clay6 tutors use Telegram* chat app to help students with their questions and doubts.
Do you have the Telegram chat app installed?
Already installed Install now
*Telegram is a chat app like WhatsApp / Facebook Messenger / Skype.