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I cut the source DNA with ECoRI and plasmid DNA with HIndII and add DNA ligase and insert it into Ecoli. But Ecoli fails to express my gene of interest because

( a ) lacks selectable marker gene
( b ) lacks antibiotic resistance gene
( c ) The restriction enzymes were different
( d ) lack of origin of replication
Can you answer this question?

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Ans: (C) The restriction enzymes were different
Same restriction enzyme has to be used for cutting at a particular site both in source DNA and Plasmid DNA
answered Nov 21, 2016 by suganyadevi.p

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