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In the B-galactosidase assay: recombinant transformed cells are ___ colored while non-recombinant transformed cells are __ color

( a ) colorless,blue
( b ) blue, colorless
( c ) blue-white,colorless
( d ) colorless,pink
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1 Answer

(A) is the right answer.

Due to presence of the insert, the galactosidase gene is disrupted and it will not be able to cleave the chromogenic sustrate, hence will appear colorless. If the gene of interest is not present, the galactosidase activity is intact and hence cleaves the substrate to produce blue colonies.
answered Mar 24, 2014 by pady_1

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