# Protoplasts from different varieties or species can be fused with the help of

$\begin{array}{1 1}(A)\;\text{Electrofusion}\\(B)\;\text{Polyethyl glycol(PEG)}\\(C)\;\text{Sodium chloride}\\(D)\;\text{Both (A) and (B)}\end{array}$

Protoplasts from different varieties or species can be fused with the help of electrofusion and polyethyl glycol(PEG)
Hence (D) is the correct answer.