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Protoplasts from different varieties or spices can be fused with the help of

$\begin{array}{1 1}(A)\;\text{Electrofusion}\\(B)\;\text{Polyethyl glycol}\\(C)\;\text{Sodium chloride}\\(D)\;\text{Both (A) and (B)}\end{array}$

Protoplasts from different varieties or spices can be fused with the help of polyethyl glycol.
Hence (B) is the correct answer.