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A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonucle

A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

1 Answer

Plasmid is a circular DNA. When an endonuclease acts on it, the DNA will become linear. It does not fragment further so that a single band is formed on the agarose gel. When a linear DNA is cut by an endonuclease, it will form two  fragments. Hence, two bands will appear on agarose gel.
answered Jul 10 by pady_1
 

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