Arrange the following in sequential order of their usage in recombinant DNA technology.

Question

$\begin {array} {1 1} (1)\;Calcium \: chloride & \quad (2)\;DNA\: ligase \\ (3)\;Ethylene \: diamine\:  tetra \: acetic\:  acid & \quad (4)\;Restriction \: endonuclease \end {array}$

          (A)     (B)     (C)     (D)

(1)     (I)      (IV)     (III)      (II)

(2)     (IV)     (I)      (II)      (III)

(3)      (I)     (IV)     (II)      (III)

(4)     (IV)    (III)     (II)       (I)