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Q)

If the polylinker sequence of the expression plasmid contain a BamHI site, 5'NGGATCCN3' and the sequence of two codons (GAT and CCN), which code for dipeptide (Asp Pro), is in frame with the fusion protein, then which chemical agent can be used to cleave the polypeptide ?


( A ) none
( B ) formic acid
( C ) boric acid
( D ) cyanogen bromide

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