Recent questions in Biotechnology and its Applications

Bt toxin is acquires the active form from an inactive protoxins at

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GM rice is enriched with

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Before a biotechnology product reaches the market, it has to undergo __ after its synthesis

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The presence of bubbles in a bioreactor

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Theromo stable DNA polymerase is isolated from the organism

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Multiple copies of a gene of interest are synthesised in vitro by

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DNA is agarose gel electrophoresis migrates towards the __

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The last step in the procedure to isolate a DNA irrespective of the source of the DNA would be

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DNA is interwined with proteins called

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Match the following

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The gene gun method employs high velocity particles of ____

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Bacterial cells can be rendered competent by exposure to ___ a.divalent cations b. monovalent cation c. either monovalent or divalent cation d. microinjection e. biolistics

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DNA is tranposted into the cell by

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___ from A.tumefaciens transformed a normal cell to cancerous one

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In the B-galactosidase assay: recombinant transformed cells are _ colored while non-recombinant transformed cells are color

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A gene for hyaluronic acid production is inserted in the ampicillin resistance region of pBR322 plasmid. The recombinant colonies will be _ resistant while the non-recombinant transformed cells are resistant

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Normal E.coli cells are resistant to

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Which of the following is true about the the origin of replication: a. responsible for the replication of any piece of DNA attached to it b. controls the copy number of the inserted gene c.it is the sequence of DNA from where transcription begins

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which of the following are commonly used as clonign vectors

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Arrange A, B, C, D and E with the smallest first:

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The DNA needs to stained by __ in order to be visualised under UV

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In gel electrophoresis, the fragements are seperated based on their

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DNA fragments are __ charged

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I cut the source DNA with ECoRI and plasmid DNA with HIndII and add DNA ligase and insert it into Ecoli. But Ecoli fails to express my gene of interest because

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the resultant sticky ends are joined by

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Overhanging regions of DNA that has been acted upon by restriction is enzymes are called sticky ends as they form __ with the complementary strand

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The restriction enzymes

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Autonomously replicating circular extrachromosomal DNA is called a __

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Multiple identical copies of a template DNA are made by

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in order to become a part of the chromosome, the alien DNA must possess

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____ are called molecular scissors

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We can avoid the insertion of undersirable gene by

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