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Recent questions tagged biotechnology: principles and processes

What would happen when one grows a recombinant bacterium in a bioreactor but forgot to add antibioti

asked Jul 10, 2017 by pady_1

1 answer

A mixture of fragmented DNA as electrophoresed in an agarose gel. After staining the gel with ethidi

asked Jul 10, 2017 by pady_1

1 answer

How does one visualize DNA on agarose gel?

asked Jul 10, 2017 by pady_1

1 answer

Describe the role of $CaCl_2$ in the preparation of competent cells?

asked Jul 10, 2017 by pady_1

1 answer

A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonucle

asked Jul 10, 2017 by pady_1

1 answer

A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuc

asked Jul 10, 2017 by pady_1

1 answer

Both a wine maker and a molecular biologist who has developed a recombinant vaccine claim to be biot

asked Jul 10, 2017 by pady_1

1 answer

What is meant by gene cloning?

asked Jul 10, 2017 by pady_1

1 answer

What modification is done on Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning ve

asked Jul 10, 2017 by pady_1

1 answer

Name a recombinant vaccine that is currently being used in vaccination program. 

asked Jul 10, 2017 by pady_1

1 answer

While doing a PCR, 'denaturation' step is missed. What will be its effect on the process? 

asked Jul 10, 2017 by pady_1

1 answer

What is the significance of adding proteases at the time of isolation of genetic material?

asked Jul 10, 2017 by pady_1

1 answer

Restriction enzymes could not have more than one site of action in the cloning site of a vector. Com

asked Jul 10, 2017 by pady_1

1 answer

What does 'competent' refer to in competent cells used in transformation experiments?

asked Jul 10, 2017 by pady_1

1 answer

What does 'H' in 'd' and 'III' refer in the enzyme Hind III?

asked Jul 10, 2017 by pady_1

1 answer

Would you choose an exonuclease while producing a recombinant DNA molecule? 

asked Jul 10, 2017 by pady_1

1 answer

How is the copy number of the plasmid vector related to the yield of recombinant protein?

asked Jul 9, 2017 by pady_1

1 answer

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