Recent questions tagged ch11

In its polyadenylated form, RNAI is highly unstable and is therefore unable to prevent formation of ______ (RNAII) , the primer for plasmid DNA synthesis.

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E. coli used as hosts for colEl plasmids carry a wild-type version of a gene known as ------ which codes for poly(A) polymerase.

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plasmid vectors which carry the origin of DNA replication from the genome of a single-stranded filamentous bacteriophage such as M13 or f1 are called

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Insertional inactivation is not possible with pUC vectors, which carry only one antibiotic resistance gene that is -

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At the origin of replication, synthesis of plasmid DNA depends on the formation of which persistent complex?

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The plasmids which replicate under stringent control require ongoing synthesis of which protein for replication ?

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When the bacterial cells are exposed to chloramphenicol, then the content of which DNA increases ?

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Plasmids, which replicate in a "relaxed" control, continue to duplicate , (a) when protein synthesis is inhibited by amino acid starvation or by (b) addition of an antibiotic such as chloramphenicol.

asked Apr 3, 2014 by pady_1

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Protein synthesis is required for (a) initiation of each round of host DNA synthesis but (b)not for plasmid replication.

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Plasmid pSC101 are said to replicate under -

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X-gal substrate is also known as -

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When X -gal is included in the agar medium, colonies carrying (a) parental non recombinant plasmids become deep blue, whereas (b) recombinant plasmids remain an ordinary creamy white or become tinted in pale egg-shell

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Bacterial colonies containing non recombinant plasmids are able to hydrolyze non fermentable, chromogenic substrates called as -

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When a segment of foreign DNA is cloned into the multiple cloning site of pUC vectors, the sequence encoding the amino-terminal fragment is disrupted. Thus, Bacterial colonies containing recombinant plasmids inhibit-

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Which plasmid vector has polycloning site consists of a tandem array of unique cleavage sites for 13 restriction enzymes: HindIIl, Spill, Pstl, SaiL Acd, HitlelI, Xiln!, BmnHI, Sma!, Xmal, Kpl1I, Sad, and EcoRl ?

asked Apr 3, 2014 by pady_1

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pUC vectors and many of their derivatives carry a short segment of E.coli DNA that contains the regulatory sequences of the lacZ gene which codes for

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The first generation plasmid namely pBR322 has two selectable markers are -

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The first plasmid used as cloning vector was:

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The word "Plasmid" was described as an extra chromosomal genetic element which was introduced by :

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Which genetic unit of plasmid is defined as the smallest piece of plasmid DNA that is able to replicate autonomously and maintain normal copy number

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Which of the following is not required for Rec-A dependent recombination between two DNA molecules ?

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Temparate bacteriophages differ from other bacteriophages because temperate bacteriophages can

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Which of the following properties is common to all cytoskeletal motor proteins (such as kinesins, myosins and dyneins) ?

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Which of the following is an advantage of confocal microscopy over conventional fluorescence microscopy ?

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Heat shock proteins are also known to act as

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Isoelectric focusing can be combined with SDS-PAGE to obtain very high resolution separations in a procedure known as

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Expand MALDI-TOF

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In which chromatography technique, molecules are separated on the basis their size and shape ?

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Visualization of proteins in living cells has been revolutionized by the discovery of which protein found in the jelly fish Aquorea victoria ?

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When gram negative bacteria is acted upon by lysozyme, in an isotonic medium, it causes rod shaped bacteria to assume spherical shape. This phenomena is due to

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Virus mediated transfer of the genetic material from one bacterial cell to the other by means of virus particles is called

asked Apr 3, 2014 by pady_1

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