Recent questions tagged ch11

Find the equation of an ellipse that satisfies the following conditions: Major axis on x-axis, passes through the points $(4,3)$ and $(6.2)$

asked Apr 3, 2014 by balaji.thirumalai

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The source of avian reverse transcriptase is

asked Apr 3, 2014 by pady_1

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Which enzyme can be used in-vitro with an oligo(dT) primer to synthesize single-stranded cDNA from mRNA templates ?

asked Apr 3, 2014 by pady_1

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Higher temperatures may be required to denature template or target DNAs that are rich in

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All thermostable DNA polymerases require __________ for activity.

asked Apr 3, 2014 by pady_1

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In a standard PCR, the amount of deoxynucleoside triphosphates (dNTPs) i.e. dATP, dTTP, dCTP, and dGTP are of

asked Apr 3, 2014 by pady_1

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The RFLPs are generated in various ways: 1. loss of an endonuclease cleavage site, most probably as a result of a base change 2. by insertion or deletion of a stretch of DNA; 3. by variation in the number of repeats of a microsatellite

asked Apr 3, 2014 by pady_1

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Szostak & Blackburn (1982), developed the first vector which could be maintained as a linear molecule, thereby mimicking a chromosome, by cloning yeast telomeres into a YRp. Such vectors are known as

asked Apr 3, 2014 by pady_1

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The need of Needleman-Wunsch and Smith-Waterman algorithm is

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Which programming algorithm is used to compare, a nucleotide sequence against a nucleotide sequence database, or an amino acid sequence against a protein sequence database ?

asked Apr 3, 2014 by pady_1

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Elaborate SCOP

asked Apr 3, 2014 by pady_1

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SWISS-PROT and TrEMBL are databases of

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Elaborate the primary sequence database (DDBJ)

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With reference to primary sequence database, the nucleotide sequence database is maintained by

asked Apr 3, 2014 by pady_1

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The use of PCR-based technique for the rapid amplification of cDNA ends (RACE) is

asked Apr 3, 2014 by pady_1

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Which DNA sequences are reverse transcribed from mRNA that reveal expression profiles in different cell types, developmental stages, and in response to natural or experimentally simulated external stimuli ?

asked Apr 3, 2014 by pady_1

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The optimum temperature for ligation of nicked DNA is

asked Apr 3, 2014 by pady_1

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Reactions catalyzed by the enzymes of E. coli and T4-infected E. coli are very similar, they differ in their cofactor requirements. (a)The T4 enzyme requires ATP, while (b) the E. coli enzyme requires NAD+.

asked Apr 3, 2014 by pady_1

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E. coli and phage T4 encode an enzyme, which seals single-stranded nicks between adjacent nucleotides in a duplex DNA chain is known as

asked Apr 3, 2014 by pady_1

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Restriction enzymes with the same sequence specificity and cut site are known as

asked Apr 3, 2014 by pady_1

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Which restriction enzyme produce blunt or flush ends like, (CCC/GGG) ?

asked Apr 3, 2014 by pady_1

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Which restriction enzyme, makes single-stranded breaks four bases apart in the opposite strands of its target sequence so generating fragments with protruding 5' termini and hence, fragments with sticky or cohesive ends ? 5'-G 5'-AATTC-3' 3'-CTTAA-5' G-5'

asked Apr 3, 2014 by pady_1

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Which restriction enzyme was discovered by Smith & Wilcox, 1970 from Hemophilus influenzae strain Rd and has cleavage sites in phage T7 DNA ?

asked Apr 3, 2014 by pady_1

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In order to apply PCR methodology to the study of RNA, the RNA sample has to be converted to cDNA to provide the DNA template for the thermostable polymerase. This process is called as

asked Apr 3, 2014 by pady_1

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Taq DNA polymerase was derived from which thermophilic bacterium ?

asked Apr 3, 2014 by pady_1

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The use of liposomes as a transformation or transfection system is called

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Which process involves the transfer of electrophoresed protein bands from a polyacrylamide gel on to a membrane of nitrocellulose or nylon, to which they bind strongly ?

asked Apr 3, 2014 by pady_1

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The pET vectors are a family of expression vectors that utilize which promotor to regulate synthesis of cloned gene products ?

asked Apr 3, 2014 by pady_1

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If the polylinker sequence of the expression plasmid contain a BamHI site, 5'NGGATCCN3' and the sequence of two codons (GAT and CCN), which code for dipeptide (Asp Pro), is in frame with the fusion protein, then which chemical agent can be used to cleave the polypeptide ?

asked Apr 3, 2014 by pady_1

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Which chemical is used for fusing $\alpha$-galactosidase-insulin A chain and $\alpha$-galactosidase - insulin-B chain ?

asked Apr 3, 2014 by pady_1

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Which gel electrophoresis method is preferred method to measure the size of an insert in a high-capacity vector ?

asked Apr 3, 2014 by pady_1

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CTAB stands for -

asked Apr 3, 2014 by pady_1

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Polyacrylamide gels cannot be cast with ethidium bromide because

asked Apr 3, 2014 by pady_1

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Which buffers serve three purposes, they increase the density of the sample, ensuring that the DNA sinks evenly into the well and add color to the sample ?

asked Apr 3, 2014 by pady_1

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Which buffers are mixed with the samples before loading into the slots of the gel ?

asked Apr 3, 2014 by pady_1

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What causes a decrease in the negative charge of the double-stranded DNA and an increase in both its stiffness and length?

asked Apr 3, 2014 by pady_1

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Molecules of double-stranded DNA migrate through gel matrices at rates that are inversely proportional to

asked Apr 3, 2014 by pady_1

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Agarose is a linear polymer composed of alternating residues of -

asked Apr 3, 2014 by pady_1

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For all organisms, from bacteria to humans, PFGE is used to study

asked Apr 3, 2014 by pady_1

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PFGE stands for ?

asked Apr 3, 2014 by pady_1

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Agarose gels have a lower resolving power than___________________, but they have a greater range of separation.

asked Apr 3, 2014 by pady_1

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During which process, the velocity of the DNA fragments decreases as their length increases and is proportional to electric field strength ?

asked Apr 3, 2014 by pady_1

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The location of DNA within the gel can be determined directly by staining with low concentrations of fluorescent intercalating dyes, such as

asked Apr 3, 2014 by pady_1

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EDTA stands for -

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During which process, the bacterial suspesion opens the cell wall, denatures chromosomal DNA and proteins, and releases plasmid DNA into the supernatant ?

asked Apr 3, 2014 by pady_1

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The exposure to an electrical charge destabilizes the membranes of E. coli and induces the formation of transient membrane pores through which DNA molecules can pass is known as -

asked Apr 3, 2014 by pady_1

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The treatment of bacteria with ice-cold solutions of CaCl2 and then heated to 37$^{\circ}$C or 42$^{\circ}$C is the process or method called as

asked Apr 3, 2014 by pady_1

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PEG stands for -

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What is SDS and its use?

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What is REBASE ?

asked Apr 3, 2014 by pady_1

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