Recent questions in Biotechnology and its Applications

Match the following:

asked Jun 4, 2020 by meena.clay6

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Which one of the following is most suitable medium for culture of Drosophila melanogaster?

asked Jun 4, 2020 by meena.clay6

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Natural genetic engineer is

asked Jun 4, 2020 by meena.clay6

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Bt gene which produces protein toxic to insect larvae is

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Gene targeting is commonly called

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Electroporation is used for

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In order to obtain virus free plants through tissue culture, the best method is

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The first restriction endonuclease was isolated by

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To which of the following techniques, DNA probes can be hybridized with RNA fragments.

asked Jun 4, 2020 by meena.clay6

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Microbes found to be very useful in genetic engineering are

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Assertion: Transgenic plant production is an application of plant tissue culture <br> Reason: an organism that contains and expresses a transgene is called transgenic organism.

asked Jun 4, 2020 by meena.clay6

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Assertion: In recombinant DNA technology, human genes are often transferred into bacteria or yeast <br> Reason: Both bacteria and yeast multiply very fast to form huge population , which expresses the desired gene.

asked Jun 4, 2020 by meena.clay6

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Assertion: In Plant tissue culture, somatic embryos can be induced from any plant cell. Reason: Any viable plant cell can differentiate into somatic embryos.

asked Jun 4, 2020 by meena.clay6

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Assertion: Nitrogen fixing enzyme in legume root nodules functions at low oxygen concentration <br> Reason: Low oxygen concentration

asked Jun 4, 2020 by meena.clay6

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Assertion: Flavr savr a transgenic tomato remains fresh and retains its flavor for long time. <br> Reason: Production of polygalacturonase enzymes which degrades pectin was blocked in Flavr savr

asked Jun 4, 2020 by meena.clay6

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The technique used for detecting specific target DNA is called

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Hybridomas are the fusion product of

asked Jun 4, 2020 by meena.clay6

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Which of the following enzymes is used to remove protein stains from the clothes?

asked Jun 4, 2020 by meena.clay6

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Small proteins produced by vertebrate cells, naturally in response to viral infections and those inhibit multiplication of viruses are called

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Monoclonal antibodies are used in

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To identify an individual by DNA analysis of their blood, investigators look for

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Human insulin is being commercially produced from transgenic species of

asked Jun 4, 2020 by meena.clay6

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Production of a human protein in bacteria by genetic engineering is possible because

asked Jun 4, 2020 by meena.clay6

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The second step in most of the genetic engineering experiments is

asked Jun 4, 2020 by meena.clay6

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Producing a ‘Giant mouse’ in the laboratory was possible through

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Which of the following is used as microbial insecticide?

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Vaccines produced by recombinant DNA technology are known as

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Which of the following can be controlled by using biopesticides?

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In a polymerase chain reaction, a synthetic sequence of nucleotides are involved in

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Insect resistance transgenic cotton has been produced by inserting a piece of DNA from

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The source of avian reverse transcriptase is

asked Apr 3, 2014 by pady_1

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Which enzyme can be used in-vitro with an oligo(dT) primer to synthesize single-stranded cDNA from mRNA templates ?

asked Apr 3, 2014 by pady_1

1 answer

Higher temperatures may be required to denature template or target DNAs that are rich in

asked Apr 3, 2014 by pady_1

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All thermostable DNA polymerases require __________ for activity.

asked Apr 3, 2014 by pady_1

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In a standard PCR, the amount of deoxynucleoside triphosphates (dNTPs) i.e. dATP, dTTP, dCTP, and dGTP are of

asked Apr 3, 2014 by pady_1

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The RFLPs are generated in various ways: 1. loss of an endonuclease cleavage site, most probably as a result of a base change 2. by insertion or deletion of a stretch of DNA; 3. by variation in the number of repeats of a microsatellite

asked Apr 3, 2014 by pady_1

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Which enzyme is an ideal target for chemotherapy because this enzyme is active in most tumors but inactive in most normal cells ?

asked Apr 3, 2014 by pady_1

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Szostak & Blackburn (1982), developed the first vector which could be maintained as a linear molecule, thereby mimicking a chromosome, by cloning yeast telomeres into a YRp. Such vectors are known as

asked Apr 3, 2014 by pady_1

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The need of Needleman-Wunsch and Smith-Waterman algorithm is

asked Apr 3, 2014 by pady_1

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Which programming algorithm is used to compare, a nucleotide sequence against a nucleotide sequence database, or an amino acid sequence against a protein sequence database ?

asked Apr 3, 2014 by pady_1

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Elaborate SCOP

asked Apr 3, 2014 by pady_1

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SWISS-PROT and TrEMBL are databases of

asked Apr 3, 2014 by pady_1

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Elaborate the primary sequence database (DDBJ)

asked Apr 3, 2014 by pady_1

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With reference to primary sequence database, the nucleotide sequence database is maintained by

asked Apr 3, 2014 by pady_1

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The use of PCR-based technique for the rapid amplification of cDNA ends (RACE) is

asked Apr 3, 2014 by pady_1

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Which DNA sequences are reverse transcribed from mRNA that reveal expression profiles in different cell types, developmental stages, and in response to natural or experimentally simulated external stimuli ?

asked Apr 3, 2014 by pady_1

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The optimum temperature for ligation of nicked DNA is

asked Apr 3, 2014 by pady_1

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Reactions catalyzed by the enzymes of E. coli and T4-infected E. coli are very similar, they differ in their cofactor requirements. (a)The T4 enzyme requires ATP, while (b) the E. coli enzyme requires NAD+.

asked Apr 3, 2014 by pady_1

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E. coli and phage T4 encode an enzyme, which seals single-stranded nicks between adjacent nucleotides in a duplex DNA chain is known as

asked Apr 3, 2014 by pady_1

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Restriction enzymes with the same sequence specificity and cut site are known as

asked Apr 3, 2014 by pady_1

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